Brew a strong cup of coffee, preferably espresso, and allow it to cool completely. This ensures the coffee is suitable for soaking the ladyfingers later.
In a large mixing bowl, combine the egg yolks and ¼ cup (50 g) of sugar. Beat the mixture at medium-high speed using a stand or hand mixer for about 5 minutes. The result should be a thick, pale mixture that forms ribbons when the whisk is lifted, indicating it is adequately aerated.
To this yolk mixture, add the chilled mascarpone and vanilla extract. Mix at medium speed for roughly 3 minutes until the blend achieves a creamy yet sturdy consistency. The mixture should hold its shape when lifted with a spoon but remain pliable enough to spread.
In a separate clean bowl, whip the egg whites using clean whisks until frothy. Gradually add the remaining ½ cup (100 g) of sugar while continuing to beat. After approximately 5 minutes, the egg whites should form stiff peaks that maintain their shape when the whisk is removed. Be cautious not to over-whip, as this can cause the whites to become grainy.
Carefully fold half of the beaten egg whites into the mascarpone mixture. Use a gentle folding motion to preserve the airiness. Once incorporated, add the remaining egg whites and fold until the mixture is smooth and uniform. Avoid overmixing to prevent deflating the mousse-like texture.